黄瓜嫩果皮色基因QTL定位分析

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黄瓜嫩果皮色基因QTL定位分析
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作者单位:中国农业科学院蔬菜花卉研究所/农业部园艺作物生物学与种质创制重点实验室，北京 100081,中国农业科学院蔬菜花卉研究所/农业部园艺作物生物学与种质创制重点实验室，北京 100081,中国农业科学院蔬菜花卉研究所/农业部园艺作物生物学与种质创制重点实验室，北京 100081,中国农业科学院蔬菜花卉研究所/农业部园艺作物生物学与种质创制重点实验室，北京 100081,中国农业科学院蔬菜花卉研究所/农业部园艺作物生物学与种质创制重点实验室，北京 100081,中国农业科学院蔬菜花卉研究所/农业部园艺作物生物学与种质创制重点实验室，北京 100081
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基金项目:国家科技支撑计划(2013BAD01B04)国家重点研发计划(2016YFD100204)

QTL Mapping of Immature Fruit Basal Color in Cucumis sativus L.

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Vegetable and Flower Research Institute of Chinese Academy of Agricultural Sciences/Key Laboratory of Horticultural Crop Biology and germplasm creation，Ministry of Agriculture，Beijing 100081,Vegetable and Flower Research Institute of Chinese Academy of Agricultural Sciences/Key Laboratory of Horticultural Crop Biology and germplasm creation，Ministry of Agriculture，Beijing 100081,Vegetable and Flower Research Institute of Chinese Academy of Agricultural Sciences/Key Laboratory of Horticultural Crop Biology and germplasm creation，Ministry of Agriculture，Beijing 100081,Vegetable and Flower Research Institute of Chinese Academy of Agricultural Sciences/Key Laboratory of Horticultural Crop Biology and germplasm creation，Ministry of Agriculture，Beijing 100081,Vegetable and Flower Research Institute of Chinese Academy of Agricultural Sciences/Key Laboratory of Horticultural Crop Biology and germplasm creation，Ministry of Agriculture，Beijing 100081,Vegetable and Flower Research Institute of Chinese Academy of Agricultural Sciences/Key Laboratory of Horticultural Crop Biology and germplasm creation，Ministry of Agriculture，Beijing 100081

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摘要:

以黄瓜(Cucumis sativus L.)嫩果皮绿色自交系‘1613’(P1)和嫩果皮白色皮自交系‘JD7’(P2)为试验材料构建重组自交系(RIL)群体(142?个株系),对各自交系嫩果皮色进行表型鉴定,利用重测序技术对各自交系进行基因分型,结合表型和基因型数据进行QTL 定位研究。结果F1代黄瓜嫩果皮颜色表现为绿色,并将控制嫩果皮颜色的基因定位到黄瓜第3号染色体上,在4.2?M的区段内定位到成簇分布的三个位点,它们在连锁图谱上的位置分别为1.01?cM、3.31?cM和6.10?cM处,与两翼标记的连锁距离分别为1.01?cM/0.09?cM,0.21?cM/0.29?cM,0.11?cM /0.19?cM。通过生物信息学分析预测出16?个候选基因,其中Csa3G904080,Csa3G904100,Csa3G903500和Csa3G902950极有可能是调控黄瓜果实颜色的关键基因。

Abstract:

A set of recombinant inbred lines (RILs) population containingS142Slines was constructed by using an inbred line with green immature fruit as female parent (1613, P1) and an inbred line of white immature fruit as male parent (JD7, P2). The phenotypic investigation for each RIL on the immature fruit skin color was performed at commodity maturity and the genotypic identification of each RIL was carried out with sequencing technology. The immature fruit of F1 generation is green peel, and three QTLs controlling the immature fruit color were located on cucumber chromosome 3, with the positions on the genetic map of 1.01cM、3.31cM and 6.10cM and the flanking genetic distance of1.01cM/0.09cM,0.21cM /0.29cM,0.11cM /0.19cM,respectively. Bioinformatics analysis founds 16 candidate genes in the target region. The gene Csa3G904080,Csa3G904100,Csa3G903500 and Csa3G902950 are very likely to be the key gene that regulates the color of cucumber fruit.

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窦文文,沈镝,麦尔旦·努尔阿伍提,等.黄瓜嫩果皮色基因QTL定位分析[J].植物遗传资源学报,2018,19(6):1138-1142.

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收稿日期:2018-01-22
最后修改日期:2018-03-12
录用日期:2018-05-03
在线发布日期: 2018-11-14
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