Using high yield cultivated soybean as recurrent parents, we made the backcrosses with intermediate materials which were derived from the cross combinations between wild and cultivated soybeans. Three new elite germplasms were developed, with protein contents above 45%, and the total of protein and oil contents above 63%. Longpin8802-1, one of the three elite germplasms, is resistant to phytothora rot and frogeye leaf spot, and its protein content, oil content and total of both protein and oil content are 45.64 %, 18.42 % and 64.06%, respectively. Longpin01-757 is resistant to frogeye leaf spot, and its protein content, oil contents and total of both protein and oil contents are 45.99%, 19.40% and 65.39%, respectively. Longpin9501 has the medium resistance to frogeye leaf spot , and its protein content, oil content and total of both protein and oil content are 45.11%, 18.32 % and 63.43 %, respectively. The data suggest that it is very effective way to broaden soybean genetic basis and develop the new soybean germplasms with high quality and resistance to diseases and good agronomic characters, using high yield cultivated soybeans as recurrent parents to make the backcrosses with interspecific enhancement.