SBPC transcription factors are mainly involved in many aspects of plant growth and development, signal mediation, and adversity stress, etc. In this study, we screened the differentially expressed gene GmSBPC with RNA-seq data of physiological microspecies stress of soybean cyst nematode No. 3 of Black Agro37 (sensing) and Dongnong L10 (resisting), and carried out bioinformatics on the spatial structure of the protein encoded by the gene, the physicochemical properties of the protein and the affinity of the gene Bioinformatics analysis. GmSBPC was cloned from disease-resistant Dongnong L10 cDNA and constructed by overexpressing pCAMBIA3300-GmSBPC in Agrobacterium tumefaciens K599 for soybean hairy root infestation. Recombinant pCAMBIA1302-GmSBPC was analysed for subcellular localisation. Nematode soils were planted with Dongnong L10 (resistant) and Dongnong 50 (susceptible), and roots, stems, and leaves were taken from SCN stress treatments on days 0, 3, 6, 9, 12, and 15, respectively, to analyse gene expression patterns by qRT-PCR. The results showed that GmSBPC protein encoded 146 amino acids, an insoluble protein, with 28.08% of α-helical region; 15.75% of elongated structure and 56.16% of irregular curls. Overexpression of hairy roots reduced the number of nematodes per unit area compared with wild-type soybean; subcellular localisation results showed that the gene was localised in the nucleus of the cell, the Under SCN stress, the expression patterns of Dongnong 50 and Dongnong L10 roots were firstly increased and then decreased, and the overall expression level of Dongnong L10 roots > Dongnong 50 roots, and the highest expression was found in Dongnong L10 roots at 12 days, which was the J₂ period of nematode infestation in soybeans. Therefore, we determined that this gene was responsive to nematode stress, and we assumed that this gene was involved in the stress response of SCN. These results provide a basis for further understanding of the multiple functions of the GmSBPC gene in stress response, and these findings help to further explore the physiological functions of the SBPC gene in soybean stress tolerance.