向日葵种质资源的耐盐性评价与筛选
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作者单位:

1.中国农业科学院油料作物研究所;2.内蒙古自治区巴彦淖尔农牧业科学院

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基金项目:

向日葵种质资源精准鉴定项目(19221985);国家特色油料产业技术体系(CARS-14-1-17);油料作物优异种质资源发掘鉴定与创新利用(CAAS-OCRI-ZDRW-202203)


Salt tolerance evaluation and screening of sunflower germplasm resources
Author:
Affiliation:

1.Oil Crops Research Institute of Chinese Academy of Agricultural Science,Wuhan;2.Bayannur Academy of Agricultural and Animal Husbandry Sciences,Bayannur

Fund Project:

The Project of Accurate Identification of Sunflower Germplasm Resources (19221985), China Agriculture Research System of MOF and MARA (CARS-14-1-17), and the Exploration, Identification and Innovative Utilization of Excellent Germplasm Resources of Oil Crops (CAAS-OCRI-ZDRW-202203)

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    摘要:

    为了解向日葵种质资源的耐盐性,鉴定发掘向日葵耐盐优异种质,本研究对444份种质资源分别进行了芽期(250 mmol/L NaCl)和苗期(250 mmol/L NaCl)耐盐鉴定和全生育期实际盐碱地种植观察,通过计算芽期发芽率、苗期存活率、叶面积、株高、SPAD值等6个指标和全生育期株高、葵盘直径、结实率、相关产量等7个指标的相对值(各指标与对照处理下的相应比值)进行耐盐性分析评价。结果表明苗期两两指标间均呈极显著正相关,相关系数在0.518-0.790之间;全生育期7个指标中多数指标间的相关性都达到了显著或极显著水平;基于这些指标进行相关性分析、主成分分析、隶属函数值计算、耐盐性综合评价和聚类分析,筛选出芽期耐盐材料132份、苗期耐盐材料9份、全生育期耐盐材料41份 ,其中6份材料(“ZX0365”、“ZX0389”、“ZX1391”、“ZX1394”、“ZX3089”、“ZX3094”)在3个时期均表现为耐盐以上等级,综合耐盐性表现好,可作为耐盐基因挖掘和耐盐品种选育等后续利用的种质材料。对这些材料3个时期(芽期、苗期、全生育期)的耐盐等级进行相关性分析,得出各时期的耐盐性鉴定结果极显著相关,芽期和苗期的耐盐鉴定结果可为实际盐碱地种植选择耐盐材料提供有效参考。本研究为向日葵耐盐品种选育提供了方法和材料基础。

    Abstract:

    In order to assess and evaluate the differences of salt tolerance among sunflower germplasm accessions, in this study, 444 sunflower accessions were evaluated for salt tolerance at seed germination and seedling stage with 250 mmol/L NaCl, and salt tolerance experiments were carried out in saline-alkali soil conditions through out the whole growth period. The relative values of germination rate at germination stage, 6 indexes (survival rate, leaf area, plant height, SPAD value, fresh weight of above-ground part and fresh weight of underground part) at seedling stage and 7 indexes (plant height, sunflower diameter, seed setting rate, seed kernel rate, 100 grain weight, grain weight per plant, equivalent yield per 667m2) at whole growth stage were determined, and the salt tolerance was analyzed and evaluated. The results showed that there was a highly significant positive correlation between each two indexes at seedling stage, with correlation coefficients ranging from 0.518-0.790. The correlation between most of the 7 indicators in the whole growth period reached a significant or extremely significant level; Based on these indexes, the analysis of correlation, principal component, membership function value, cluster and comprehensive salt tolerance evaluation were performed, and 132 salt resistant materials at germination stage, 9 at seedling stage, and 41 at whole growth stage were screened. Six accessions ("ZX0365", "ZX0389","ZX1391","ZX1394","ZX3089","ZX3094") showed above salt tolerance grade in the three periods with good comprehensive salt tolerance, which can be utilized for subsequent salt-tolerant gene mining and salt-tolerant variety breeding. The correlation analysis of the salt tolerance grades of these materials at the three stages showed that the identification results of each stage were significantly correlated, indicating that salt tolerance assessments at the seedling and germination stages can serve as effective references for the planting selection of salt-tolerant materials in saline-alkali soil. This study provided method and material basis for the selection of salt-tolerant sunflower varieties.

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  • 收稿日期:2023-11-30
  • 最后修改日期:2023-12-20
  • 录用日期:2024-01-05
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