In order to identify the pre-harvest sprouting resistance of wheat germplasm resources, effective molecular markers for pre-harvest sprouting resistance were screened and excellent white grain wheat germplasm resources for pre-harvest sprouting resistance were excavated. In this study, pre-harvest sprouting resistance of 222 wheat germplasm resources was identified by whole-pre-harvest sprouting test in laboratory, and the functional molecular markers of 8 anti-pre-harvest sprouting genes, including myb10D, DFR-B, Vp1B3, PM19-A1, MFT-3A, MFT-A2, MKK3-A, QSD1, were used to detect the genotypes of the tested materials. Phenotypic identification results showed that there were significant differences in the indoor relative pre-harvest sprouting rate of 222 wheat germplasm resources. The relative pre-harvest sprouting rate ranged from 0.00 to 1.15, and the average relative pre-harvest sprouting rate was 0.73. 38 wheat were identified as resistant to pre-harvest sprouting, including 9 white wheat, 27 red wheat and 2 black wheat. Correlation analysis between allele types and relative pre-harvest sprouting rate showed that functional markers myb10D, DFR-B, Vp1B3, MFT-3A and MFT-A2 were significantly correlated with relative pre-harvest sprouting rate, while PM19-A1, MKK3-A and QSD1 were not significantly correlated with sprout resistance. These results indicated that molecular markers myb10D, DFR-B, Vp1B3, MFT-3A and MFT-A2 could be used for detection of pre-harvest sprouting resistance and marker-assisted breeding. Based on the results of phenotypic and genotype detection, nine white wheat germplasm resources with pre-harvest sprouting resistance were selected, including Yunong 914, Yunong 946, FengDecunmai 30, Fanmai 5, Xumai 029, Lianmai 1901, Baofeng 1903, Zhengmai 829 and 13wang27-8, which could be used for genetic breeding and variety layout of wheat pre-harvest sprouting resistance.