Using artificial inoculation technique， a total of 166 sorghum male-sterile and maintainer lines which introduced from the countries of the USA， India， Australia and China， were evaluated for resistance against the diseases of sorghum target leaf spot （TLS） and sorghum anthracnose （SA） at 2019 and 2020. The results showed 26 accessions highly resistant （HR） to target leaf spot accounting for 15.66%. Thirty-four accessions rated as resistance （R） to TLS accounted for 20.48%， and 42 accessions rated as moderately resistance （MR） to TLS accounted for 25.30%. Forty-six and 18 accessions， which were rated as susceptible （S） and highly susceptible （HS） to TLS， accounted for 27.71% and 10.84% respectively. There were eight accessions rated as highly resistant （HR） to sorghum anthracnose accounting for 4.82%. Eighty-four and 56 accessions rated as resistance （R） and moderately resistance （MR）， accounted for 50.60% and 33.73%， respectively. There were 14 accessions rated as susceptible（S） and 4 accessions rated as highly susceptible （HS） to SA， accounting for 8.43% and 2.41%， respectively. The results indicated that there was no resistance difference between sorghum male-sterile and maintainer lines to TLS. And there was no resistance difference between most male-sterile and maintainer lines to SA， except for Tx2790， 91NF18 and KSP335. The proportion of the USA resistance sources to TLS and SA was much larger than the other countries. Therefore， the introduction and improvement of foreign germplasm resources should be strengthened. Thirty-eight accessions were found being simultaneously resistant to both TLS and SA， including 20 American accessions， 16 Chinese accessions and 2 Indian accessions. These resistant sources used in sorghum breeding should aid in expanding the resistance gene diversity and in the development of new resistant varieties.