Genetic stable female sterile line is the key to realize heterosis utilization, it is also a essential pathway to achieve simplified hybrid seed production by using female sterile gene. A female sterile line in genetics, named FS2106, was first discovered during our investigation of germplasm resource. The phenotypic traits, pollen viability and chromosome numbers were tested in the plants, while two hybrid combinations and their segregation populations were built with FS2106 and other two normal female parent, thus traits separation of female sterile, stigma color, shenth color and involurce texture were observed in F₁ and F₂generation populations, and inheritance modes and regulations of four traits were revealed by χ₂ ^test. The results showed that plant height, stem diameter, tillers, leaf length and leaf width were 86.9 cm. 33.4 per plant, 4.9 mm, 34.3 cm and 2.2 cm in average, respectively. In flowering stage, its pistillate spikelet grows without development, but tassels are proper growth and produce viability pollens, exhibiting stable female sterile characteristics. Chromosome number was a normal type of 2n=20 as well. Moreover, the female sterile trait in FS2106 is a recessive nuclear inheritance controlled by a single gene, while its purple shenth and stigma and involurce texture are the dominant heredity of single gene, but alleles were heterozygous in involurce texture loci. In conclusion, the discovery of FS2106 and the mining of female sterility gene could provide the basic materials for the heterosis utilization and seed production of adlay, and traits of shenth, stigma and involurce texture can be acted as macroscopic flags in breeding procedure.