LBD gene family is a kind of plant-specific transcription factors and plays very important role in plant development. In this study, 59 LBD genes distributed on 9 chromosomes of radish genome were identified. The radish LBD genes have simple structures with the intron numbers being less than 3. These LBD genes could be classified into 2 classes (Class I and Class II) according to the phylogeny relationship, including 50 and 9 members, respectively. The number of radish LBDs distributed on different chromosomes has significantly difference, 18 members distributed on chromosome 1 while only one member distributed on chromosome 7 and 8, respectively. The expression patterns of radish LBD genes in different tissues and development stages showed spatial and temporal differences, uncovered their potential diverse function in radish development. These results will be helpful for the functional analysis of the radish LBD genes family.