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  • 袁海静,袁汉民,刘飞,赵兴增,武小阳,董立国,刘兰英,张维军,何进尚,王晓亮.宁夏野生枸杞(Lycium barbarum L.)苦味性状研究[J].植物遗传资源学报,2017,18(5):991-1000.    [点击复制]
  • .Study on bitter taste character of wild Ningxia wolfberry in China[J].植物遗传资源学报,2017,18(5):991-1000.   [点击复制]
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宁夏野生枸杞(Lycium barbarum L.)苦味性状研究
袁海静,袁汉民,刘飞,赵兴增,武小阳,董立国,刘兰英,张维军,何进尚,王晓亮
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(宁夏农林科学院国家枸杞工程技术中心 银川;宁夏农林科学院农作物研究所银川;江苏省中国科学院植物研究所 南京 中国农业科学院研究生院 北京;中国农科院研究生院;宁夏农林科学院荒漠化治理研究所 银川)
摘要:
本研究采用植物学分类、常规杂交、PCR-SSR分子标记检测、超高效液相色谱-高分辨质谱[UPLC-MS(HRMS)]等方法,围绕宁夏野生苦味枸杞的苦味性状进行了研究。结果表明:野生苦味枸杞花丝、花柱的长度、种籽大小和颜色等方面与其它中国枸杞种质资源存在较大差异,其花蕾中的雄蕊花丝、雌蕊花柱呈弓曲状,多数情况下其浆果簇状着生。PCR-SSR分子标记检测结果表明,宁夏野生苦果枸杞DNA扩增出了栽培枸杞品种宁杞1、2、3、4、5、7号等所没有的条带;在111-147bp之间,扩增出了略高于中国枸杞其它种质资源的条带,其分子量高于中国枸杞其它种质资源相应的DNA的分子量,遗传差异明显。以宁杞7号做母本与宁夏野生苦味枸杞杂交,所得到的F1代成熟浆果出现苦味、甜味分离个数比例接近1:1。宁杞7号做母本与野生半苦味枸杞杂交的F1代浆果苦、甜味的分离个数比例为1:4。其反交F1代浆果均为苦味。野生苦味枸杞苦味基因可能是杂合的。苦味性状可能受多个显性加性基因控制、并与细胞核、质遗传物质互作有关。而甜味性状可能受隐性基因控制。初步判定,苦味物质是具有二氢槲皮素结构的黄酮类化合物。
关键词:  簇状浆果  杂交亲和性  苦味物质  二氢槲皮素
DOI:10.13430/j.cnki.jpgr.2017.05.023
投稿时间:2016-12-02修订日期:2017-07-27
基金项目:科技部科技基础性工作专项(2011FY110200)、宁夏农林科学院科技创新先导资金项目(NKYQ-13-11)。
Study on bitter taste character of wild Ningxia wolfberry in China
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Abstract:
The bitter taste character of Ningxia wild bitter wolfberry was studied, using methods of botany classification, conventional hybridization, PCR-SSR molecular marker detection, Ultra performance liquid chromatography high resolution mass spectrometry?[UPLC-MS(HRMS), and other in this paper. The results showed that, comparing with other Chinese wolfberry germplasm, the length of filament and style, seed size and color etc. of Ningxia wild bitter wolfberry had larger differences. Its stamen filaments and pistil style of flower buds were curly shape. Most berries clustered. PCR-SSR molecular marker detection results showed that DNA of Ningxia wild bitter wolfberry was amplified a band, which hasn ‘t found it in cultivativars that Ningqi 1, 2, 3, 4, 5, 7, etc..Menwhile, betwenm 111bp and 147bp, Ningxia wild bitter wolfberry DNA amplified a band which was slightly higher than other Chinese wolfberry germplasm resources, It means that the molecular weight of the site was higher than other germplasm resources of Chinese Lycium barbarum L. It had a significant difference in genetic material. As a female parent, Ningqi 7, a major cultivar in Ningxia, crossed by Ningxia wild bitter wolfberry, the mature berries of hybrid F1 generation had a proportion of bitterness and sweetness separation, which closed to 1:1. Meanwhile, the separation ratio of bitter to sweet berries had 1:4 in F1, when Ningqi 7, as female parent, crossed by wild semi bitter wolfberry. However, all of F1 fruits were bitter from the reverse cross. Obviously, bitter genes may be heterozygous of wild bitter wolfberries in rural environment.However, it indicated that bitter traits might be controlled by multiple dominant genes with additive genetic effects, and it might relate to the interaction between nuclear and plastid genetic material. The sweet trait might be controlled by recessive genes. According to preliminary determination, the main source of bitter substance might be flavonoids with Taxifolin structure.
Key words:  Cluster Berry  Cross Compatibility  Bitter Taste Substance  Dihydroquercetin

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