Wheat Cultivar “Weimai 8” is a good source of adult plant leaf rust resistance in China. In order to understand the chromosomal position of leaf rust resistance genes, 179 F2:3 families of the cross Weimai 8 and Zhengzhou 5389 (susceptible to leaf rust) were used to map leaf rust QTL. Results showed that there was a major QTL on chromosome 2AS, temporarily named QLr.hbau-2AS. QLr.hbau-2AS provided by the resistant parent Weimai 8, was between SSR markers Xcfd36 and Xbarc1138, interval length was 2.58 cM, in 2010-2011, 2011-2012 and 2012-2013 crop season, explained 25.79%, 71.55% and 60.72% of the phenotypic variation, respectively. This experiment screened 13 molecular markers linked to QLr.hbau-2AS, these molecular markers will play an important role in molecular breeding in the future.