应用SSR标记分析鹰嘴豆种质资源遗传多样性
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SSR Markers Analysis Genetic Diversity of Chickpea (Cicer arinuml L.) Germplasm
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    摘要:

    种质资源的遗传多样性是育种工作的基础,本研究利用SSR标记对鹰嘴豆资源进行遗传多样性,旨在发掘鹰嘴豆资源中丰富的遗传变异。从48对鹰嘴豆SSR引物中筛选出18对核心多态性引物,对96份不同来源的鹰嘴豆种质资源进行SSR标记的遗传多样性分析。结果表明,18对SSR引物共检测到115个等位变异,占总检测位点的52.99%,每对SSR引物可检测出3~10个等位变异,平均6.39个;平均每个位点多态信息量(PIC)为0.8107,变化范围为0.6661~0.8984。Shannon′s信息指数平均为1.4769,变化范围为0.0607~1.9584。UPGMA聚类结果表明,在遗传相似系数0.59处,可将96份鹰嘴豆资源分为6个类群,类群Ⅰ包含9份资源,类群Ⅱ包含2份资源,类群Ⅲ包含28份资源,类群Ⅳ包含4份资源,类群Ⅴ包含40份资源,类群Ⅵ包含13份资源。本研究为对我国鹰嘴豆种质资源的评价与利用、优异基因的挖掘、育种亲本材料的选择等提供科学依据。

    Abstract:

    Genetic diversity of germplasm resources was the foundation of thebreeding study.SSR markers analysis genetic diversity of chickpea (Cicer arinuml L.) germplasm.18 polymorphic SSR markers which was screened from a total of 48 SSR primer pairs detected 115 allelic variations which were 52.99% of total alleles for diversity in 96 chickpea accessions.The average number of alleles per SSR were 6.76 with a range from 3 to 10. The polymorphic information content (PIC) of each SSR locus ranged from 0.6661 to 0.8984 with an average of 0.8107.Shannon′s information index varied from 0.0607 to 1.9584 with an average of 1.4769.UPGMA cluster analysis showed that the 96 chickpea materimals were clustered into six categories at the genetic similarty coefficient of 0.59.The categoryⅠhad 9 varieties,Ⅱhad 2 varieties,Ⅲ had 28 varieties, Ⅳ had 4 varieties,Ⅴhad 40 varieties and Ⅵ had 13 varieties。Genetic diversity of the chickpea germplasm resources based on SSR markers was abundant. The study can provided reference for chickpea resources evaluation , utilization and exploration ofexcellent gene resources. The genetic diversity research of chickpea germplasm resources has guiding significance for breeding material parent.

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张金波,苗昊翠,王威,等.应用SSR标记分析鹰嘴豆种质资源遗传多样性[J].植物遗传资源学报,2015,16(5):1027-1034.

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  • 收稿日期:2014-08-18
  • 最后修改日期:2015-07-14
  • 录用日期:2015-07-15
  • 在线发布日期: 2015-09-15
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