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  • 范英明,张登荣,于大德,张炎,董明亮,张鸿景,张金凤.河北省华北落叶松河北群体遗传多样性分析[J].植物遗传资源学报,2014,15(3):465-471.    [点击复制]
  • Fan Yingming,Zhang Dengrong,Zhang Jinfeng.Genetic diversity and population structure of Larix principis rupprechtii Mayr in Hebei Province[J].植物遗传资源学报,2014,15(3):465-471.   [点击复制]
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河北省华北落叶松河北群体遗传多样性分析
范英明,张登荣,于大德,张炎,董明亮,张鸿景,张金凤
0
(北京林业大学;四川省西昌市凉北林业局;河北省林业科学研究院)
摘要:
以来自河北8个群体共312个华北落叶松天然个体为材料,利用10对SSR引物对群体进行扩增,所得数据用于其遗传多样性和遗传结构的研究。结果表明:10对SSR位点共检测到42个位点,平均每个位点有4.2个等位基因,位点的等位基因数(Na)为2-6个。群体平均等位基因数为3.36,Shannon指数(I)平均为0.749,观察杂合度Ho为0.405、期望杂合度He为0.423,来自河北北部的围场台子水和吉字以及兴隆雾灵山的多样性水平最高,南部的阜平群体多样性水平最低。AMOVA分子差异分析显示3%的遗传变异存在于群体间,97%的遗传变异则存在于群体内,基因分化系数Gst仅为0.028,表明华北落叶松在河北的群体分化很低。聚类结果表明河北围场两个群体为一类,蔚县、隆化和涿鹿为一类,阜平和赤城为一类,兴隆雾灵山群体距离其它较远。多样性北部群体较高,南部较低,表明华北落叶松可能的衍化方向是从北向南,印证前人研究北部可能是起源中心。
关键词:  华北落叶松  SSR分子标记  遗传多样性  种群结构
DOI:
投稿时间:2013-10-26修订日期:2014-01-16
基金项目:北京市与中央在京高校共建项目“华北落叶松遗传资源收集评价及新品种选育”(GJ2011-2); 国家林业局林业公益性行业专项 “重要乡土树种核心种质评价及高效育种共性技术研究”(201004009)
Genetic diversity and population structure of Larix principis rupprechtii Mayr in Hebei Province
Fan Yingming,Zhang Dengrong,,,,,Zhang Jinfeng
(Sichuang Liangbei Forestry Administration)
Abstract:
Based on three hundred and twelve samples of eight natural Larix principis rupprechtii Mayr populations in Hebei province of North China, ten pairs of SSR primers were utilized to study the genetic diversity and population structure of these populations. A total of 42 alleles were detected,with a mean of 4.2 bands per primer, and the alleles of each locus (Na) were 2-6. The mean allele of population was 3.36,the mean Shannon’s information index (I) was 0.749,and the observed heterozygosity and expected heterozygosity were 0.405 and 0.423,respectively. Among the eight populations,Taizishui and Jizi from Weichang county and Wuling mountain populations which were all located in the north of Hebei had the richest genetic diversity,while fuping population had the poorest one.The AMOVA analysis showed that the percentage variation among the populations was 3%,while the percentage variation within the populations was 97%,and Gst was only 0.028, illustrating that the genetic differentiation was quite low. Clustering result showed that,the two Weichang populations were clustered into one group,the Yuxian, Longhua and Zhuolu populations were clustered into another group,and the Fuping population and Chicheng population were clustered into one group. The Wuling population was far from the others. The north population have a higher diversity than south, it means than the Evolution direction was from north to south, and north may be the center of origin.
Key words:  Larix principis rupprechtii Mayr  SSR molecular marker  genetic diversity  population structure

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