Abstract: The meiosis process of pollen mother cell, the pollen grains vitality of L.lancifolium from Hanzhong district,Shanxi province, was observed. The results showed that the length of buds and anthers, and the colour of the anther wall were related to the stage of the meiosis process: The length of buds and anther which were in the meiosis process were between 2.5 and 3.2 cm /1.4 and 1.8cm,respectively.The main chromosome configurations were trivalent in diakinesis stage, and the average configuration was 11.6Ⅲ+2Ⅱ+1.1Ⅰ,which showed that the L.lancifolium was used in this experiment is autotriploid. The asymmetric division, chromosome lag, chromosome bridge and micronucleus were present in distinct meiosis stages, which provided the cytological evidence for the low fertility of pollen grain. The prospect of application of tripolid L.lancifolium in the Lily breeding was also discussed in this article.