In order to test the verity of hybrids derived from the cross of salt tolerant and salt sensitive cultivars,three polymorphic SSR markers between soybean parents were selected to evaluate 148 hybrids.The purity ratio of soybean hybrids is 81.8%,and the results identified by different SSR markers were identical.This study indicated,if the parents were pure lines,that a single appropriate SSR marker can be used to test seed purity of the hybrids.The SSR loci with great fragment length different between the parents should be selected in order to identify the genotypes by agarose gel.In order to estimate the effectiveness of this method,fifty seeds of a hybrid line named H02-286 were used as materials for further research,and the result showed the feasibility of this method.