Identification of six litchi putative clones with 20 arbitrary primers of 10 bases were carride out by random amplified polymorphic DNA(RAPD)markers.Each of them had the same 125 bands,which affirmed that they were high similar to type of Guangming.The genetic relationship of the 43 litchi germplasm resources were analyzed by RAPD with 30 arbitrary primers of 10 bases.212 RAPD loci were polymorphic and accounted for 80.83% of all the loci.The 43 accessions could be classified into 4 groups at the level of the similarity coefficient of 78%,and Guangming belonged to Group 3.These groups were generally with traits of maturing periods.No characteristic band was found to identify Guangming from other germplasms,but it can be identified from others by a combination of several primers such as AP09,AM16 and AK17.