Molecular Detection and Identification of Xanthomonas albilineans Causing Leaf Scald of Sugarcane in Zhanjiang, Guangdong Province of China
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Affiliation:

1.Scientific Research Institute of Zhanjiang State Farms;2.National Engineering Research Center for Sugarcane,Fujian Agriculture and Forestry University;3.South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Science,Guangdong Zhanjiang

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Fund Project:

Earmarked Fund for the China Agricultural Research System of MOF and MARA(CARS-170302,CARS-170708)

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    Abstract:

    To clarify the bacterial pathogen causing sugarcane leaf scald in west of Guangdong province, we conducted field surveys of leaf scald regarding 27 newly-introduced sugarcane varieties in two calendar years (2019 and 2020). For 15 sugarcane stalk samples of suspected diseased plants,isolating the pathogen(s)and molecular identification by PCR were further performed. Based on the results of field trials,eleven sugarcane varieties were found with the typical symptom of leaf scald. Among them,the diseased plants from four varieties GT08-1589,DZ12-88,FN11-2907,and FN09-7111 were found in both the plant cane and ratoon cane,with incidence rates from 0.2% to 9.4%. The bacteria isolated from 15 diseased stalks were detected by PCR with the specific primers XAF1/XAR1 of Xanthomonas albilineans. These fragments are 608 bp in length and share 100% sequence identity to the published nucleotide sequences of X. albilineans isolates in China,USA,and France. These isolates were clustered in the same clade(PFGE-B). Altogether,our results confirmed that leaf scald occurred in 11 cultivars from Zhanjiang sugarcane-planting region was caused by X. albilineans.

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History
  • Received:November 12,2021
  • Revised:November 22,2021
  • Adopted:December 06,2021
  • Online: May 10,2022
  • Published:
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