Orange wheat blossom midge（OWBM，Sitodiplosis mosellana Géhin） is an important pest insect that seriously threatens wheat production. Cryptic habitat of OWBM lifecycle made pest control very difficult. Therefore，screening for OWBM-resistant wheat germplasm and tightly-linked molecular marker is of great important for pest control. In this study，566 wheat germplasm resources from different wheat regions and different periods，and genomic regions surrounding major OWBM locus were used to identify their resistance to OWBM，and to discover new SSR markers，respectively. The results showed that 27.03% of wheat cultivars were immune or highly resistant to OWBM，of which released in early years were still resistant to OWBM. Meanwhile，565 SSR markers were developed from the genomic region of major OWBM-resistant locus（Qsm. hbau-4A），and their detection efficiency were tested among this panel of wheat accessions. Of these SSR markers， marker Xhs87201bp showed robust PCR amplification and presented an average of 65.37% detection efficiency，and can be used for marker-assisted selection of OWBM resistance.