Through interspecific hybridization, the gene for the trait of yellow skin of the fruit, which exists only in Cucurbita pepo, was transferred to C. moschata and C. maxima. Genetic analysis showed that the inheritance pattern in interspecific progenies was in line with the Mendelian law. Genetic performances of the yellow skin gene were compared among the three species in Cucurbita. The results showed that trait expression of this gene was affected by multiple factors including species, parental fruit shape and color. New Cucurbita germplasms with novel yellow skin can be created by transferring the gene from C. pepo to C. moschata and C. maxima through interspecific hybridization.