Genome-wide Analysis of GRAS Transcription Factors in Ricinus communis and Response to Abiotic Stresses
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Inner Mongolia University for Nationalities

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The National Natural Science Foundation of China(31460353), Inner Mongolia Key Laboratory of Castor Breeding Fund Project (MDK2018017,MDK2018018), Inner Mongolia University for Nationalities Autonomous Region Science and Technology Reserve Project(2018NDCB05)

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    Abstract:

    GRAS transcription factors play important roles in many physiological and biochemical pathways involved in plant growth and development and stress responses. 48 GRAS transcription factors were identified in the genome of Ricinus communis L., and their physical and chemical properties, phylogenetic patterns, gene structure, and conserved motifs were analyzed. The proteins encoded by GRAS genes of castor are all hydrophilic, with acidic proteins accounting for about 94%, isoelectric points between 4.82-10.21, and relative molecular weights between 17305.2-90986.6. The GRAS transcription factors are divided into 11 subfamilies,and members of the same subfamily have similar gene structures and conserved motifs. RT-qPCR was used to detect the expression of five genes in roots, stems and leaves under drought and salt stresses. The results showed that the expression of RcGRASs in different tissues was specific, and drought and salt stresses induced the expression of RcGRAS14、RcGRAS21 and RcGRAS35 but inhibited the expression of RcGRAS1 and RcGRAS10. This study provides a reference for further study of the functionon of GRAS transcription factors in abiotic stresses.

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History
  • Received:May 21,2019
  • Revised:June 23,2019
  • Adopted:July 17,2019
  • Online: January 17,2020
  • Published:
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