Genotyping And Genetic Structure Analysis for 235 Tea Plant (Camellia sinensis var. assamica cv. Mengku-dayecha) in Shuang-jiang of Yun-nan Province
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1Tea Research Institute, Chinese Academy of Agricultural Sciences/National Centre for Tea Improvement,, Hangzhou 310008;2Yunnan Shuangjiang Mengku Tea Co., Ltd. Shuangjiang 677302

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Earmarked Fund for China Agriculture Research System (CARS-19); the Key Scientific and Technological Project for New Variety of Tea Plant Breeding of Zhejiang Province (2016C02053).

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    Abstract:

    Camellia sinensis var. assamica (Assam type), which served as special germplasm resource, were widely distributed and cultivated in geographical and ecological environments of the Yun-nan province. Due to redundant phenotypic variations, these materials become of importance in studies of tea evolution and breeding. The cultivar (cv.) Mengku-dayecha (MK tea) is a national sexual variety originated from Meng-ku town, Shuang-jiang County of Yun-nan province, and this genotype shows the characteristics of strong bud, leaf fertility and tenderness. MK tea becomes popular and is widely cultivated in the western and southern tea -producing counties of Yun-nan province. Within this study, 235 germplasm resources of MK tea plants, which were collected from Shuang-jiang county of Yun-nan province, were subjected to evaluate genetic diversity and genetic relationships among individuals, construct molecular fingerprints, select suitable core marker combinations, analyse population genetic structure. The results showed that: the polymorphism of each SSR marker had detected in the candidate samples. The alleles of markers ranged from 2 to 7 with mean value 3.84, and Ho ranged from 0.18 to 0.74 with mean value 0.47; the genotyping combinations by 25 SSR loci had confirmed to accurately identify each sample and each of them had been given a unique fingerprint. Indeed, 8 core SSR loci were successfully screened out as a simplified combination which had only 8.1 e-5 to 7.7 e-3 probability to appear the same genotype combination in the sample group; 235 samples collected from 11 regions were divided into 5 sub-populations (R, G, Y, Pi, Pu) and 3 groups (Pu + Y, R+G, Pi + Y) based on genetic structure analysis. Taken together, we proposed that R sub-population in ML and BD area was likely derived from the south area (NL), while group Pu + Y and group Pi + Y highly associated and originated from Y population. Notably, the genetic components of Pu and Pi populations assumed to be caused by the migration of tea resources in Dong-guo area.

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History
  • Received:October 22,2018
  • Revised:May 06,2019
  • Adopted:December 04,2018
  • Online: July 16,2019
  • Published:
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