Stable Transformation of Haloxylon ammodendron HaNAC1 Gene to Improve Drought Resistance of Potato
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Agricultural Bio-Technology Centre, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan, 750002

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Nation Science Foundation Project(31560053), Ningxia Agriculture Breeding Special Project(2014NYYZ01), Ningxia Academy of Agriculture and Forestry's 13th Five-Year Major Scientific Research Project (YES-16-0102), Ningxia Science Foundation Project (NZ16123, NZ16122, NZ17124,NZ14210), Ningxia Academy of Agriculture and Forestry Science and Technology Innovation Pilot Fund Project (NKYJ-17-03, NKYJ-17-07, NKYJ-17-19,DWHZC-2017002)

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    Abstract:

    NAC transcription factors are involved in plant abiotic stress response and play important roles for improving plant drought and salt tolerance. Taking advantage of established agrobacterium-mediated transformation platform using the potato cultivar "Atlantic", we generated the transgenic potatoes by expressing Haloxylon ammodendron HaNAC1, and conducted tests for drought resistance with PEG-6000 simulated drought treatment. By analyzing the contents of endogenous hormones, these results showed significant increased content of auxin (IAA, IBA), cytokinin (IP, cZ), jasmonic acid (MEJA, JA-ILE), salicylic acid (MESA), gibberellin (GA3) in three transgenic potato lines if compare to the receptor line (p<0.01, p<0.05). The content of abscisic acid (ABA) was significantly lower than the receptor line. By analyzing the transcriptional level using quantitative PCR analysis, several stress-tolerant genes including NCED1, ERD11, RD29A, DREB2A, LEA3 and KIN1 showed the modified expression in HaNAC1 overexpressing plants to the receptors (p<0.01, p<0.05). Taken together, our results suggested that increased drought resistance by expressing HaNAC1 is associated with the different expression of stress-related genes and hormone content, thus providing a theoretical basis for further molecular mechanism analysis of HaNAC1 response to drought stress.

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History
  • Received:October 15,2018
  • Revised:April 11,2019
  • Adopted:January 15,2019
  • Online: July 16,2019
  • Published:
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