Identification of Candidate Genes for Lobed-leaf Trait in Brassica napus L. by SLAF-seq Method
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The Crops Institute of Jiangxi Academy of Agricultural Sciences/Jiangxi Research Station of Crop Gene Resource & Germplasm Enhancement,Ministry of Agriculture.P.R. China,Nanchang 330200

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Creation and genetic analysis of determinate inflorescence germplasm in Brassica napus(31561004418);Accurate indentification, creation and utilizatiaon of the excellent germplasm resources of Brassica napus(2016FYD0100202-17);Creation and genetic improement of determinate inflorescence germplasm in Brassica napus(S2016NYZPF0201);The fine mapping and gene clone of the leaf-lobed characterics of Brassica napus L.(20141513010002);The chief expert and breeding post of the rapeseeds industrial system in Jiang Xi province (JXARS-08)

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    Abstract:

    Lobed-leaf is widely known as an ideal morphological marker in rapeseed breeding. Genetic variation of lobed-leaf plays an important role in enriching the leaf shape diversity and improving genetic breeding of rapeseed. In this study, fifty plants with normal leaf and 50 plants with lobed-leaf were selected from the F2 population respectively, which was derived from a cross of the normal leaf line Zhongshuang No.11 and the leaf-lobed new line Y176. Two bulked DNA pools were generated, which were subjected for SLAF-seq-BSA (specific-locus amplified fragment sequencing and bulked segregant analysis). A total of 177,941 labels were obtained and 150,168 SNPs were identified, including 128,200 SNPs that were polymorphic between both parents. A physical interval expanding ~2.83 Mb on chrA10 (14,528,149~17,353,693 bp) was tightly associated with lobed-leaf trait. Within that, 666 candidate genes was found. Since the genetic confidence interval of this locus differed to the physical positions of previously reported genes, there might be a new gene controlling leaf margin cleavage. Furthermore, 666, 517, 587, 151 and 258 genes in this region were annotated by NR, Swiss-Prot, GO, KEGG and COG databases, respectively. Thus, this information might be helpful in future map-based gene isolation of lobed-leaf in Brassica napus L.

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History
  • Received:August 14,2018
  • Revised:January 12,2019
  • Adopted:November 23,2018
  • Online: March 19,2019
  • Published:
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