Identification of Cabbage(Brasisica oleracea. var. Capital L.) Club Root Pathogen (Plasmodiophora brassicae. Wor.)and Assessment of Germplasm
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Agricultural ministry National modern agriculture industry technology system project(CARS-25); Jiangsu science and technology support project(BE2013429);Jiangsu Agricultural science and technology innovation fund projects[CX(13)2004]

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    Abstract:

    Morphological identification and PCR confirmed that the Plasmodiophora brassicae is the pathogen caused cabbage clubroot. Using European ECD identification system, the?pathogen?was?further?identified?as a?physiological?strain coded as ECD17/31/13.?Field?trial?of?88?cabbage?germplasm?artificially?inoculated?by?ECD17/31/13?was?conducted?in village Huoshaoping(HShP),?Hubei?Province,?to?assess?their?resistance?nature?to?the?clubroot. After?evaluation,? their resistant phenotypes?were?leveled?as?high?resistance?(HR),?resistance?(R),?tolerance?(T) and susceptible(S).?The?results?showed?that:? among?the?88?cabbage?germplasm?engaged,?one?was?HR,?seven?R?and?seventeen?T?in?seedling?stage,?and?four?HR,?four?R,?15?T? in?plant?stage.?Germplasm?CR21?was?the?HR?material?which?performed?the?best?in?both?stages?stably.?Materials?CR55?and?CR54?were?found?as?the?most?susceptible.?The?disease?index?of?CR55?and?CR54?were?76.19?and?75.97?in?seedling?stage,?and?74.10 ?and?81.54?in?plant?stage?respectively,?when?the?incidence?rates?of?CR55?and?CR54?were?100%?both.?The?taxonomic?and?virulence?evaluation?on?the?pathogen?of?clubroot?disease,?and?screening?resistant?germplasm?in?cabbage?would?bring?clue?to?further?understand?the?phytopathological?mechanism?underlying?and?material?for?plant?breeding.

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History
  • Received:January 25,2016
  • Revised:September 19,2016
  • Adopted:June 20,2016
  • Online: November 07,2016
  • Published:
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