To study of forming mechanism of fruit stoneless, 4CL genes in full length with 1819bp coding sequence encoding protein of 591 amino acids was obtained from Ziziphus jujuba Mill.‘Wuhexiaozao’ by the PCR technology. It is named as Zj4CL (accession number KP279930).The alignment of Zj4CLand genomic sequence found that,the genome contains 5 exons and 4 introns.Sequence alignment and motif analysis showed that Zj4CLgene included Box I and Box II of 4CL gene families. Protein sequence alignment showed it had a highest genetic relationship with Malus domestica (XP_008362825). The results of qRT-PCR revealed that the highest expression levels were appeared on the 30th day after flowering. The expression 40th day after flowering declined apparently.