ABSTRACT: Chinese Cymbidium has extremely high ornamental value. Owing to the lack of its genomic resource, research on its genetic background has been limited. The gene-derived markers (genic markers) have a high transferability, which make them an idea molecular marker for genetic studies across related species. Also, the genic markers could be used to explore the relationship between Chinese cymbidium’s genetic and geographic background. Here, a total of 62 genic-SSR makers in our lab were used for genetic analysis. The results exhibited that 52 were polymorphic when cross-tested among 5 Cymbidium species comprising 43 accessions. The genetic distance averaged 0.389, ranging from 0.016 to 0.600. The polymorphic index content (PIC) of 52 polymorphic markers averaged 0.415, ranging from 0.054 to 0.824. The phylogenetic analysis and principle component analysis (PCA) showed that the most of accessions derived from the same species were grouped together. However, C. goeringii accessions did not always form a separate cluster, suggesting that C. goeringii accessions were polyphyletic. Interdisciplinary cross-analysis of genetic and geographic background indicated that some accessions from same species were clustered into different branches based on their origins; some other accessions from near regions also clustered together, across species.