MicroRNAs constitute a recently discovered class of non-coding small RNAs, negatively regulate gene expression at the post-transcriptional leve l in eukaryotes. A large number of studies show that miRNAs play important roles in regulating many biological approaches. Bioinformatics prediction and analysis of miRNAs is one of the most important strategies for the discovery and identification in plant. The research content summarizes methods and strategies of bioinformatics prediction of miRNAs and their target genes,expounds the important role of bioinformatics in the study of plant miRNAs and is a foundation for future researches.