The colors of petal and anther collected from 40 mango cultivars were digitally described and determined by using Photoshop CS to extract the parameters of CMYK pattern in the typical color region. Results showed that the percentage of C, M, Y, K could represent the colors of petal and anther more accurately and scientifically, and reflect differences between varieties of mango. They were divided into seven and four groups by UPGMA cluster analysis. The research will provide A new digital and scientifical method for germplasm description and new cultivar breeding of mango.