Cloning and expression profiling analysis of a transcription factor ChNAC1 in hazelnut(Corylus heterophylla Fisch.)
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    Abstract:

    NAC transcription factors are a family of functionally diverse proteins. These plant-speci?c NAC domain genes play an important role in response to various stresses. A cDNA encoding the NAC-like gene homologue was isolated from hazelnut(Corylus heterophylla Fisch.)by RACE-PCR and designated ChNAC1 (GenBank Accession No. HQ639415). Sequence analysis showed that cDNA of ChNAC1 was 1154 bp long and contained a single open reading frame. The predicted ChNAC1 protein has 291 amino acids with an estimated molecular mass of 33.31 kDa and an isoelectric point of 6.66 kDa, qRT-PCR analysis showed that the expression of ChNAC1 was induced by low temperature and peaked at 4 h after exposed to low temperatures of 4℃. The transcripts of ChNAC1 appeared in many hazelnut tissues including male inflorescence, bark, ?ower bud and seeds, but mostly accumulated in male inflorescence. These results suggest that ChNAC1 may function in cold stress signal transduction pathway.

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History
  • Received:December 24,2011
  • Revised:December 24,2011
  • Adopted:December 30,2011
  • Online: February 09,2012
  • Published:
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