In the present study, four medium (CIM, W4, SD2 and MSD2) were used for callus induction from mature embryos of Yangmai 158 and Bobwhite, model varieties with high capacity in callus regeneration. Two medium (MRM, 1/2MS+5mg/L Zeatin) were used for the following callus regeneration. Both varieties had the highest regeneration frequency on callus induction medium CIM followed with the regeneration medium 1/2MS+5mg/L Zeatin. Tissue culture capacities of mature embryos from 40 Shandong elite varieties or advanced lines were evaluated. The results showed that the callus induction had no significant differences in these cultivars and lines, but the callus status and the growth speed and the following regeneration capacity were quite different. Among the 40 lines, only 08H05 had the higher regeneration frequency (23.3%) than that of the Bobwhite (21.3%), eight lines (08H02, 08H05, 08B08, Taimai20-2, Taishan5024, Liao9514, Hemai9803, Bobwhite) with more than 10% regeneration percentage, account for 20% in the survey lines, seven lines can not obtain any regenerated plantlet, accounting for 12.5%, and 25 lines account for 62.5% with the regeneration frequency no more than 10%.