Pre-harvest sprouting is the devastating disaster of wheat, but varieties resistant to the disaster is rare in common wheat. Germination rate of 35 Ae. tauschii accessions from the middle reaches of the Yellow River, 14 Ae. tauschii accessions from abroad, and 2 wheat cultivars were tested. Meanwhile，diversification analysis classfied the materials into several categories. The results indicated that all Ae. tauschii accessions have stronger pre-harvest sprouting resistance than common wheat, and those from the middle reaches of the Yellow River have stronger pre-harvest sprouting resistance than those from abroad. Among those from the middle reaches of the Yellow River, T005, T007, T008, T016, T030, T062, T065, T068, T069, T072 and T085 were identfied to have strongest resistance to pre-harvest sprouting, and could be applied in wheat breeding.