Seed setting rate,pollen fertility and development of pollen were studied in 141 accessions of Oryza.rufipogon Griff.collected from the six sampling sites in Gaozhou City,Guangdong Province.The results showed that the seed setting rate of all accessions of the wild rice was low,with the average of 57.23%.The percentage of pollen fertility was 89.3%,in which 134 accessions(accounting for 95.04%) were of over 50%,suggesting normal fertility.The pollen fertility in 7 accessions(accounting for 4.96%) was low,in which the least was 8%(GZW122).The main abnormal sterility in pollen expresses typical sterile type.Abnormal anther dehiscence happened in 17 accessions(accounting for 12.06%).Correlation analysis showed that both anther dehiscence index and pollen fertility were the factors to affect seed setting rate.The seed setting rate,pollen fertility and anther dehiscence index were different in the wide rice in different sampling sites.Among them,Xiangshan Cave,Xiangshan County,Xiangshan Town,Gaozhou City gave the least average in the seed setting rate,pollen fertility and anther dehiscence index.However,coefficient of variation was the highest in them,which indicated the high diversity in reproduction in this site.The pollen development went through eight stages,i.e.microsporocyte formation stage,microsporocye meiosis stage,early microspore stage,middle microspore stage,late microspore stage,early bicellular pollen stage,late bicellular pollen stage and mature pollen stage,which was similar to that in O.sativa L..