Abstract:In this study, 57 resistant soybean collections from both China and US were used as materials. They were detect for SNPs at rhg 1 and Rhg 4 loci by using single based chain extension with a microsphere-based assay. The purpose was to illustrate the distribution of alleles at SNPs loci in Chinese accessions and provides the information for utilization of SCN resistant accessions in China. The comprehensive resistances of Chinese soybean SCN resistant accessions were more related to the SNPs genotypes compared to resistance for each SCN race. At both rhg 1 and Rhg 4 loci, 7 of 9 US resistant accessions appeared to be resistant genotypes, and 37 of 49 had resistant genotypes too, taking 77 8% and 66 7% of the total accessions, respectively. Based on this result, it was postulated that most of the Chinese resistant accessions in this study had resistance by interaction of both rhg 1 and Rhg 4, but the other resistant accessions may have different resistant mechanism.
Abstract:本研究以H2 1×Mo17的F2 :3 群体 (12 0个家系 )为作图材料 ,利用SSR和AFLP标记对玉米资源H2 1的亚洲玉米螟抗性进行了数量性状位点 (QTL)分析。结果表明 ,基于叶片侵食度性状 ,检测到 3个QTL ,分别位于染色体 1、5、8上 ;基于茎秆虫孔数性状 ,检测到 3个QTL ,分别位于染色体 4和 10 (2个 )上 ;基于茎秆隧道长度性状 ,检测到 2个QTL ,位于染色体 4和 8上 ;以隧道长度 /虫孔数为鉴定性状 ,检测到 1个QTL ,位于染色体 4上。这些QTL所能解释的表型变异在 7 7%～5 1 8%之间。超显性是QTL作用的主要方式。
Abstract:Relationships and parental specific markers among sugarcane germplasm were analyzed with RAPD. 25 arbitrary primers with polymorphism were selected and used to make the RAPD fingerprinting of 41 sugarcane germplasm. The RAPD data were clustered using UPGMA method. It showed that genetic diversity existed among sugarcane cultivars and relative species. But the genetic similarities were high among these sugarcane germplasm, indicating their close relationships. Some specific RAPD markers were amplified from some sugarcane parental species with some certain arbitrary primers. The parental specific RAPD markers could be observed at a higher frequency in their progenies.
Abstract:Plasmid DNA with foreign chitinase gene was introduced into water melon lines by the approach of pollen-tube pathway. Transgenic plants were obtained by basta screening and PCR amplification.Three transgenic lines with improved resistance to Fusarium wilt were selected by natural occurrence and artificial inoculation. The results indicated that the chitinase had certain inhibition effect on Fusarium oxysporum, and the method of pollen tube pathway could be successfuly used in watermelon transformation.
Abstract:wheat varieties (lines) were identified for resistance to different races of Puccinia striiformis f.sp tritici, a causal agent of wheat stripe rust, in Gansu during 1994-2002. The results showed that the resistant level of winter wheat was higher than that of spring wheat and the resistance of Gansu's varieties was better than that of varieties collected from other provinces. The results in the fields indicated that the varieties released in the main wheat production areas were susceptible to stripe rust and only a few Gansu's varieties, such as Longjian 127, were resistant. Some of resistant sources used in breeding at present were resistant and some became susceptible. By artificial inoculation with different races, field monitoring for resistance to stripe rust and powdery mildew and survey of agronomic characteristics, 20 accessions were identified as good sources for breeding in the future.
Abstract:Six identical japonica rice varieties from two sources were cultivated in the field. One set was from seeds stored in the National Long-term Genebank and was used as the control, and the other was from the seeds having higher regeneration cycles and being used in the production all along. Differences between the two sources on seedling emergence rate, growth duration, agronomic traits and grain quality were studied. Deterioration in chalky quality was observed for rice of higher regeneration cycles on five of the six varieties, whereas no significant differences were shown for other quality traits. On the other hand, the number of filled grains per panicle was lower for those from the genebank, indicating that long time storage in low temperature might result in a decline of grain number. In addition, emergence rate of stored seeds was also relatively lower than that of higher regeneration cycles.
Abstract:Twenty-eight soybean accessions were selected from the National Genebank,including 19 Mancangjins from various regions,9 accessions having related pedigree.The relationships between all accessions were characterized by agronomic traits and SSR markers. Base on the data came from agronomic traits and SSR,there were obvious differences between all of 28 accessions.23 SSR primers were used and 151 alleles were detected with average 6.6 alleles per locus. Analyzing all Mancangjin's genetic relationship with Jinyuan and Huangbaozhu which are authenticatedas as the parents of Mancagjin,it is speculated that ZDD00078 and ZDD00924 are more conceivably derived from the cross between Huangbaozhu and Jinyuan.
Abstract:The powdery mildew resistance genes in 11 tetraploid wheat-Aegilops amphidiploids, Triticum carthlicum acc. PS5, Triticum durum acc. DR147, 4 Aegilops accessions and advanced lines Am9/Laizhou953*2 F 5 and (DR147/Ae14)//Laizhou953*2 F 4 were analyzed by inoculating detached primary leaf segments with a set of 20 differential powdery mildew isolates. By comparisons of the response pattern of differential wheat cultivars or lines and application of the gene-for-gene hypothesis, we deduced that resistance gene Pm4b occurred in line Am9/Laizhou953*2 F 5, and an unknown resistance gene in combination with resistance gene Pm4b occurred in T. carthlicum acc. PS5. Line (DR147/Ae14)//Laizhou953*2 F 4 and T. durum acc. DR147 possessed an unknown resistance gene in combination with resistance gene Pm4a,respectively Ae. caudata acc. Ae14 and Ae. umbellulata acc. Y39 showed resistance to all the isolates tested, they should carry a novel resistance gene because no powdery mildew resistance gene has been introgressed into common wheat from Ae. caudata and Ae. umbellulata. Except in Am9, the resistance of T. carthlicum acc. PS5 or T. durum acc. DR147 was in part suppressed in an amphidiploid, the resistance of Aegilops accession was completely, or in part, suppressed in an amphidiploid.
Abstract:Fifteen characters of cotton yield and quality were analyzed with Grey Correlation Method. Synthetic estimates on long-staple cotton lines tested was given in this paper, which provided scientific basis for approval and application of new varieties.
Abstract:Through investigation of the 29 varieties of L.esculentum in the locae germplasm repository, the results indicated that there were at least 10 pairs of morphological genic variations,which could provide the rich potential of different genetic recombination. It reflected to certain extent that the germplasm repository had the potential to preserve genetic diversity. The preliminary results through examining the fruit quality of 7 F 1 using 6 parents showed that some qualities of the F 1 would surpass those of the marketing varieties. By using 18 RAPD primers to amplify DNA of 6 parents and their 8 F 1, the results showed that 12 different primers varied in the relative ability for showing the polymorphism of the varieties. The primers that can both amplify and efficiently show the polymorphism were A11, K05, C15 and A17, in which K05 and A11 could produce most amplified bands. Furthermore, the primers A11 and C19 could be used to examine the purity of 2013B, and B11 to 2018A.
Abstract:Soybean(Glycine max)production varieties and germplasm from 14 Chinese major planting provinces in the year of 2002 have been investigated with regard to protein and fat contents . The investigation showed that the average protein and fat content of soybean germplasm appeared to be higher than those of the varieties used in production ,respectively. The range of fat content of germplasm was so broad as to exhibit superior selective advantage in soybean breeding for higher fat contents. The average protein content of the domestic soybean germplasm was higher than that of germplasm introduced from abroad, and the average fat content of germplasm from abroad was higher than the domestic germplasm .The average protein content of the soybean varieties from the Huanghuaihai ecological region seemed to be higher than that of varieties from the northern ecological region.However, the average protein content of soybean varieties used in production from the Huanghuaihai ecological region at present proved to be lower than that of newly bred testing varieties in the same region .The same varieties planted in different locations varied greatly in respect of fat contents.
Abstract:One hundred and twenty five landraces of common wheat from Tibet were evaluated for their agronomic characters. The resucts showed that the plant height of most accessions was too high to be used for breeding program and the tiller number of most accessions was less than ten. Obvious differences were found in spike length, number of spikelets and per length spikelet and some special types such as musti-spikelet types and dense spike types were included. Correlation analysis of characters shown that the increase in the number of tillers and spikelet per spike and the length of spike would cause the increase in the plant height and the decrease of 1000-grian weight. In most cases, the accessions with the longer spike length would always have more spikelet.Moreover, the utilization porential, strategies and the reason for different performance of Tibetan waeet in Dujaingyan,Sichuan also are discussed.
Abstract:This paper presents the progress of research on germplasm resources, genetic control of fiber color of naturally colored cotton. The combination of conventional breeding and biotechnology is the development mainstream for the future's naturally colored cotton, which will promote the development of fiber quality improvement and color innovation.
Abstract:Sitobion avenae(Fabricius)is a serious pest of wheat in China. Breeding and planting resistant varieties is an ideal way to control Sitobion avenae. Progress of studies on screening resistant wheat germplasm resources, resistance mechanism and effect of varieties on population dynamics of wheat aphid is reviewed. In the future, research on resistant genes against Sitobion avenae should be strengthened.
Abstract:Dactylis glomerata L. is a worldwide-cultivated species for its high yield, good quality and shade resistance. China also holds an extensive distribution of wild Dactylis. However, reports on researches on Dactylis glomerata are still rare. This review summarized the morphological, cytological and molecular diversities of Dactylis glomerata. Genetic polymorphism of Dactylis glomerata exits at all levels. It can be predicted that Systematical studies on genetic diversity of wild orchard grass rich in China should be very important to reasonable conservation, utilization and breeding of new varieties of Dactylis glomerata.