In order to explore the response of flower organ traits and lint percentage to high temperature， and to evaluation the breeding materials of Gossypium barbadense L. with high temperature tolerance， this study evaluated 5 traits （4 flower organ traits and lint percentage） of 534 Gossypium barbadense L. resources in Awati County in 2022， and then selected 36 materials with extreme traits.Statistical analysis showed that the variation coefficient of 534 Gossypium barbadense L. resources ranged from 8.57% to 26.56%.Correlation analysis revealed that style length was extremely significant positively correlated with the other 4 traits.In 2023， we conducted an enhanced temperature shed heat stress test on 36 previously selected Gossypium barbadense L. resources with extreme traits in Awati County and measured the performance of 23 phenotypic traits.The results showed that the 36 extreme resources showed different degrees of variation in agronomic traits and 14 traits showed obvious difference compared with the control.Principal component analysis identified 6 principal components， which accounted for 85.34% .Cluster analysis divides the resources into four categories： heat resistant， medium heat resistant， thermosensitive and highly thermosensitive.Correlation analysis confirmed the positive correlation between the effective boll number， the effective branch number and the heat resistance （comprehensive evaluation value）. Multiple regression analysis further showed that the goodness of fit between flower organ traits， lint percentage and pollen activity and comprehensive evaluation value was not high.This study provided important theoretical basis and germplasm resources for Gossypium barbadense L. breeding for high temperature tolerance.