1.Crop Research Institute , Gansu Academy of Agricultural Sciences, Lanzhou 730070;2.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081
Foundation projects: China Agriculture Research System(CARS-06-14.5-A8);Gansu Provincial Top-notch Talents; Gansu Provincial Science and Technology Program Project (23CXNA0036,22CX8NA028)
Broomcorn millet boasts numerous varieties and substantial genetic diversity in China, providing valuable parental resources for new varieties breeding. However, limited research has been reported on constructing core germplasm resources from this extensive resource pool. In this study, 490 broomcorn millet germplasm resources were categorized based on variety type and spike morphology. The square root proportion method was used to determine the number of samples, and cluster analysis was conducted with each group for 20 quality and quantitative traits. Combined with random sampling, a core germplasm collection was established. The representativeness of this core germplasm collection was evaluated by using mean, variance, coefficient of variation, genetic diversity and trait retention ratio, as well as histograms, principal component analysis and correlation analysis were used to determine the consistency of all germplasm and core germplasm.Results showed that the constructed core germplasm of 55 broomcorn millet lines (11.22% of total germplasm) included 26 local varieties (47.28%), 9 bred varieties (16.36%), 9 wild accessions (16.36%), and 11 foreign varieties (20.00%). Significance analysis revealed no significant differences between the core germplasm collection and the entire germplasm in genetic diversity, coefficient of variation, variance, and mean of phenotypic traits , suggesting that the established core germplasm collection effectively represents the entire germplasm.
