Identifying and mining resistance genes from germplasm resources is of great significance for molecular breeding of common bean， as few such resistance genes to wilt disease have been deployed in cultivars. By taking advantage of the former study that reported the identification of candidate resistance gene PvFWR1 （Fusarium Wilt Resistance 1） to common bean wilt， using a genome-wide association study and haplotype analysis， this study conducted further molecular characterization of PvFWR1. The PvFWR1 gene sequences from resistant genotype Tuheyaozidou and the susceptible genotype Longyundou No.3 were isolated. This gene harbors one single exon with a complete coding sequence （CDS） of 1104 bp encoding for 367 amino acids， and is annotated as a LRR-RLKs protein family member. Transcriptional analysis using RT-qPCR showed that the PvFWR1 transcripts in the root tissue of resistant genotype were 2.1-fold higher than that of the susceptible material. The expression in the root tissue of infected plants was significantly up-regulated upon pathogen inoculation. Knock-down of PvFWR1 in the resistant material using VIGS technology resulted in decreased resistance to pathogen inoculation， while overexpressing this gene in the susceptible material by deployment of the hairy root transformation method enhanced pathogen disease. Collectively， our results demonstrate that PvFWR1 is a resistance gene against Fusarium wilt in common bean， offering a foundation for elucidating the resistance mechanism and breeding use of this resistance gene.