Abstract:To provide a basis for breeding liquor-making sorghum with marker-assisted selection and exploring the genetic mechanism of key genes underlying these traits, this study used high-density genetic map to identify QTL for panicle architecture-related traits. 205 RIL population from a cross between BTx623 (American cultivar) and Hongyingzi (Cultivar for brewing Kweichow Moutai liquor) were used to investigate six panicle traits in five environments of Guiyang, Anshun and Ledong from 2020 to 2021, these traits included Panicle length (PL), panicle handle length (PHL), number of cob nodes (NCN), number of primary branches (NPB), and length of the longest primary branch length (LLPB). QTL were identified using the inclusive composite interval mapping (ICIM) method. Totally, 61 QTL were detected on 45 unique loci, of which 14、10、8、11 and 18 QTL were related to PL, PHL, NCN, NPB, and LLPB, respectively. 19 important QTL identified in multi-traits or multi-environments distributed on chromosome 1(3), 3 (4), 4 (2), 5 (1), 6 (4), 7 (1), 8 (2), and 9 (1), and 13 orthologous genes for rice panicle architecture were found in or near 12 of the 19 QTL, such as DEP1, RGN1, OsPID, OsSPL7 and WTG1, which could provide a basis for further gene cloning and function verification.