MYB is one of the largest number of transcription factors in plants. MYB is involved in a variety of physiological and biochemical processes of rice, such as root development, cell development, secondary cell wall synthesis, tillering formation, flower organ development, spike morphogenesis, seed development, stimulatory metabolism, secondary metabolism, biological stress and abiotic response. This paper introduced the classification and protein structure of MYB transcription factor family, reviewed the latest research progress of MYB family members in growth and development, hormone signals in rice, described the regulatory role of MYB in abiotic stress processes of rice, such as drought, high temperature, low temperature, high salt, UV damage, etc., and also discussed the defense role of MYB in biological stress such as fungi, bacteria, etc. Finally, based on the latest research progress of rice MYB transcription factor, deficiencies were summarized and prospects for future research were put forward.