Potato cyst nematode (PCN) is an important quarantine disease in potato, which can reduce the yield of potato by 80%.In recent years, it has been reported that the potato golden nematode (Globoderarostochiensis) has been found in several major potato producing areas in Southwest China.In order to ensure the safety of potato production in China, it is urgent to screen germplasm resources with cyst resistance and select resistant varieties.Using molecular markers N146, N195, 57R, TG689, Gro1-4-1 and Gpa2-2, 875 potato germplasm resources preserved by Yunnan Academy of Agricultural Sciences were screened for resistance genes to potato cyst nematode.211 resources were screened to contain H1 molecular markers (N146, N195, 57R and TG689) of the resistance gene of potato wireworm, of which 114 resources contained 4 molecular markers at the same time;Seven resources contain molecular marker Gro1-4-1 of potato golden nematode resistance gene Gro1-4;237 resources contain Gpa2-2 molecular marker of potato white nematode resistance gene Gpa2.The results of this study will provide technical support and material basis for the breeding of potato cyst nematode resistance.