Abstract: To evaluate the plant height, grain traits, and diseases resistance of wheat resources derived from different wheat regions of China at four major breeding stages that cultivated in Ningxia spring wheat region, and to provide feasible evaluation methods and excellent germplasms for Ningxia wheat breeding, a total of 228 domestic wheat resources belonged to four breeding stages were used. The entropy-weighted DTOPSIS method was used for comprehensive evaluation of the plant height and grain traits of these germplasms in order to select good breeding parents. The disease resistance and distribution of resistance genes of these materials was evaluated by field test combined with 18 molecular markers associated with major wheat diseases in Ningxia. The results showed that the plant height and the protein traits of materials showed the reduced whereas the grain numbers and grain weight showed increased tendency from breeding stages I to IV. Based on the Ci value, we selected a total of 11 materials belong to the top 5% of the total. These materials were Hongxinglong No.3, Yangnuomai, Xinjiang wheat, Shanglin wheat, Ningchun No.45, Huining No.19, Ningchun No.32, M6445, Ganchun No.25, Yongliang No.15 and Xiaobing No.33, respectively. Besides, these 11 materials also showed good resistance to stripe rust and leaf rust in the field test, and three of them, namely Hongxinglong No.3, Shanglin wheat, and Xiaobing No.33 showed resistance to powdery mildew. In the field, there were 181, 220 and 83 in 228 materials showed resistant to stripe rust, leaf rust and powdery mildew, respectively. Further, 18 molecular markers associated with diseases resistance including Xfa2019, Xwmc221, Wpt-7004, Xgwm18, Xcfa2040, cfd71, Xgwm293, Xgwm304, Xgwm149, Xgwm526, Xgwm332, 5EST-237, KU.962, XMAG1714, Xmag1759, Xgwm160, Xicsx79 and WGGBH218, showed amplifications in 194, 227, 23, 30, 127, 68, 57, 31, 153, 65, 211, 191, 1, 226, 225, 77, 62 and 191 of the 228 materials, respectively. In word, the DTOPSIS method based on entropy weighting and molecular marker associated with major wheat diseases can be used to evaluate the domestic wheat resources at the different breeding stages. The 11 wheat resources with good comprehensive traits can be used as candidate parents for Ningxia wheat breeding.