In order to excavate excavate dissect elite waxy maize germplasm resources from landraces and improve the utilization efficiency of waxy maize breeding，the genetic diversity，relationship and population structure was analyzed by genome-wide scan based on of 10K SNP markers among 207 Guangxi waxy maize landraces and 6 reference waxy inbred lines. The results showed that the overall genetic diversity of the Guangxi waxy maize population was relatively high，with an average expected heterozygosity (He) of 0.22，observed heterozygosity (Ho) of 0.31，and an average polymorphic information content (PIC) of 0.25. The genetic diversity in central Guangxi was the highest (He=0.24，Ho=0.32，MAF=0.24，PIC=0.26) at the population level. Landraces were divided into four major groups by population structure analysis. The genetic relationship between populations and the originations of landraces attribution of taxa taxa are not completely related to geographical location. In general, the genetic similarity coefficient among Guangxi waxy maize landraces is low, and the genetic diversity is abundantThe overall genetic similarity coefficient between landraces is low revealed the genetic diversity is rich . Analysis of variance for molecular variation (AMOVA) showed that 99% of the genetic variation derived from population per se. The genetic differentiation coefficient (FST) between groups populations populations was 0.005，indicating a low-level differentiation among groups populations. The summary results of the present study indicated These results cleared cleared the genetic diversity and relationship of waxy maize landraces in different regions of Guangxi，and provided a theoretical guidance for waxy maize germplasm improvement and breeding of new varieties in Guangxi.