The peanut pod abscission ability，which is the difficulty of pod shedding after maturity，is mainly related to the fracture force of gynophore-pod and gynophore-stem. In order to explore the variation of pod abscission among different peanut varieties，the fracture force of gynophore-pod and gynophore-stem of 294 genotypes were systematically measured and analysed at the beginning of harvest and after drying for 4 days in this experiment. The results showed that there were significant differences in fracture force of gynophore-pod and gynophore-stem among different peanut varieties. The variation of the gynophore-pod fracture force among 294 germplasms at the beginning of harvest and after drying for 4 days were 2.07-20.07 lb and 1.73-9.64 lb respectively，while the variation of the gynophore-stem fracture force was 2.21-22.86 lb and 1.90-11.25 lb respectively. The variation of gynophore-stem fracture force was observed at the beginning of harvest. The correlation between the characteristics of pod abscission and the main stem height and the number of pods per plant was not significant. Nine and 20 peanut varieties with suitable pod abscission ability after harvest and drying were screened respectively，which laid elite germplasm resource to breed new varieties of peanuts suitable for mechanized harvesting.