Cloning of SpsLAZY1a and SpsLAZY1b Gene Promoters from Salix psammophila and Their Expression Analysis
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1.Forestry College of Inner Mongolia Agricultural University;2.Ordos Forestry and Grassland Enterprise Development Center,Inner Mongolia Ordos;3.Ordos Afforestation Station, Inner Mongolia Ordos

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National Natural Science Foundation of China (31660216),Inner Mongolia Autonomous Region Applied Technology Research and Development Fund Project (2021GG0075,2019GG004),National Science and Technology Major Special Project (2018ZX08020002-005-005)

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    Abstract:

    To study the transcriptional regulation mechanism of SpsLAZY1a and SpsLAZY1b genes, their promoter fragments were cloned from Salix psammophila C. Wang & C. Y. Yang. By analyzing cisacting element in the promoter sequences using plantCARE database,the promoter sequences of both genes contain the core elements CAAT-box and TATA-box,and the elements responding to light,methyl jasmonate, abscisic acid,ethylene,gibberellin,and low temperature. GUS staining in tobacco transient expression and stable transgenic 84K poplar revealed the transcriptional activity of both promoters ProSpsLAZY1a and ProSpsLAZY1b. The ProSpsLAZY1a activity was stronger than that of ProSpsLAZY1b in transgenic 84K poplar. The results of stem basal sections of transgenic 84K poplar showed that the expression of ProSpsLAZY1a and ProSpsLAZY1b was mainly observed in endothelium and phloem. The promoters of SpsLAZY1a and SpsLAZY1b genes were non-tissue-specific,and their activities of the promoters were different. Collectively,this study provides a reference for analyzing the regulation mechanism of LAZY gene.

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History
  • Received:October 11,2021
  • Revised:December 27,2021
  • Adopted:January 14,2022
  • Online: May 11,2022
  • Published:
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