Wild relatives are natural gene pools for crop germplasm innovation and improvement. 30% of global crop yield growth is contributed by the application of crop wild relatives. However, due to habitat destruction and pollution, overuse of resources, blinded introduction of varieties and climate change, the wild relatives of crops are under threat and a proportion of natural populations and population genetic diversity have been lost. In-situ conservation is an important strategy to protect wild relatives and their nature habitats as well as protect interaction between wild relatives and environment to maintain their evolutionary potential. Based on the summary of domestic and foreign policies and technical means of in-situ conservation, this paper summarizes the technical route of in-situ conservation, puts forward the problems and working focus of in-situ conservation of crop wild relatives in China. The large data related to genome, phenotype and environment should be closely integrated with in-situ conservation of crop wild relatives, which provide strong theoretical support for the in-situ conservation of crop wild relatives.