Detection and Evaluation of Blast Resistance Genes in Exotic Rice Germplasm Resources
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Rice Research Institute, Fujian Academy of Agricultural Sciences

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Fundamental scientific research projects of public welfare scientific research institutes in Fujian Province(2017R1021-4); Investigation, Collection, Evaluation and Application of Crop Germplasm Resources in Key Agricultural Counties of Fujian Province (A2017-8); Fujian Natural Science Foundation(2016J01134); Science and Technology Innovation Team Project of Fujian Academy of Agricultural Sciences(STIT2017-1-1); Species Variety Resources Conservation Fee Project(11821301354052031).

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    Abstract:

    In order to identify new resistant resources of 156 exotic rice germplasm accessions for breeding in Fujian Province, test for rice blast resistance was conducted at two calendar years in experimental plot in Shanghang, Fujian. Genotypes carrying any of rice blast resistance genes Pi2, Pi9, Pi5, Pi54, Pikm, Pita, Pia and Pib were detected by molecular selection markers. As a result, 10, 14, 29 and 26 accessions showed resistant tseedling blast, leaf blast, neck blast and comprehensive resistance, respectively. Furthermore, the correlation analysis suggested significant positive correlations between seedling blast and leaf blast (r = 0.816, P < 0.01), seedling blast and neck blast (r = 0.347, P < 0.01), and leaf blast and neck blast (r = 0.344, P < 0.01). Marker assisted selection indicated 1, 6, 20, 22, 37, 88, 101 and 106 rice accessions, which harbored the resistance genes Pi9, Pi2, Pi54, Pikm, Pi5, Pib, Pia and Pita, respectively. 60% of accessions containing either of Pi9 and Pi2 genes were resistant to rice blast. A subset of rice accessions was found with stacking of 0 to 6 blast resistance genes. An elevation on rice blast resistance was observed if several resistance genes were present in an accession. For example, a combination of resistance genes Pi9+Pi5+Pikm+Pia, Pi5+Pib+Pita+Pikm+Pia and Pi2+Pi54+Pib+Pita+Pikm+Pia displayed the better resistance. Finally, 8 rice blast resistant genotypes were identified with potential for rice breeding.

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History
  • Received:April 14,2019
  • Revised:July 25,2019
  • Adopted:October 08,2019
  • Online: March 17,2020
  • Published:
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