The 13 agronomic characters of the 65 Coix germplasm resources collected were studied the principal component analysis and cluster analysis in this paper. The results showed that the main agronomic characters could be reduced to the 6 principal components of leaf width, plant height, stem diameter, total bract surface characteristics, total bract texture, total bract color, fructification type and 100-grain weight. And the 65 germplasm resources could be divided into three groups. The first group including 15 materials had the best comprehensive characteristic for the highest height and the largest 100-grain weight. The study on recent collection of Yunnan Coix plant resources evaluation of diversity, and provides a theoretical basis for the use of Coix germplasm heterosis and parent selection.