Expression analysis and subcellular localization of wheat aluminum-induced gene TaAIP
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    Abstract:

    TaAIP was obtained as the interaction protein of wheat stress-related TaMAPK2, which was used as bait protein to screen the wheat cDNA library by yeast-two-hybrid system. TaAIP, containing a wali domain, was similar to some aluminum-induced proteins. Real-time PCR showed that the expression of TaAIP was up-regulated by the imposition of aluminum, high-salt and drought stress. Semi-quantitative RT-PCR analysis indicated TaAIP was a stem-specific gene, not expressed in root, leaves and flower. The subcellular localization assay indicated that TaAIP located on plasma membrane. These results provided the foundation for further analysis of TaAIP resistance mechanisms.

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History
  • Received:March 19,2013
  • Revised:May 16,2013
  • Adopted:August 09,2013
  • Online: August 21,2013
  • Published:
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