Genetic analysis and mapping QTLs of amylose content were conducted using a F2 segregation population of Haomuxi/Guichao 2.The results showed that the amylose content of Haomuxi was controlled by a recessive major QTL, and it was not allelic to the wx locus. Besides the major QTL there were several minor QTLs. The major QTL was detected on chromosome 11 with LOD scores 15.4 with SSR markers, which is linked to RM224. This major QTL can explain 32% of phenotypic variation.