Cloning and sequence analysis of lectin-like receptor kinases gene in TcLr45
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Ph.D. Programs Foundation of the Ministry of Education of China

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    Abstract:

    A fragment of lectin-like receptor kinases gene was amplified in wheat leaf rust near-isogenic line TcLr45. The full length cDNA of the gene 2347bp was obtained using 3’ and 5’ RACE-PCR, based on the target sequence. This sequence had a full open reading frame, encoding 730 amino acids, named as LecRK-LR45. Software analysis showed that LecRK-LR45 had a typical structure of lectin-like receptor kinases, belonged to L type. Cluster analysis result indicated that LecRK-LR45 had a closer genetic relationship with lectin-like receptor kinases gene of Aegilops tauschii and wheat(Lr34), and farther with maize and arabidopsis. The cloning of this gene lies a foundation for researching the function of lectin-like receptor kinases in wheat.

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History
  • Received:August 15,2012
  • Revised:December 20,2012
  • Adopted:March 11,2013
  • Online: April 02,2013
  • Published:
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