Cloning and Transient Expression Assay of Two nsLTP Gene Promoters from Chimonanthus Praecox in tobacco
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The National Natural Science Foundation of China (General Program, Key Program, Major Research Plan)

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    Abstract:

    The promoter fragments of 1298bp and 838bp upstream from the 5’ upper of Chimonanthus praecox non-specific lipid transfer protein(nsLTP) gene family member CpLTP3 and CpLTP4 were isolated from the genomic DNA of Chimonanthus praecox by hiTAIL-PCR technique respectively. Bioinformatics analysis revealed that the two sequences contained basic cis-elements,such as TATA-box and CAAT-box and many elements involved in the plant abiotic stress. The result of transient expression indicated that all the sequences had the function to drive reporter gene GUS in tobacco.

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History
  • Received:September 23,2011
  • Revised:May 31,2012
  • Adopted:May 22,2012
  • Online: June 27,2012
  • Published:
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