The heavy metal-associated isoprenylated plant proteins (HIPPs), which play an important role in the maintenance of metal ion homeostasis and detoxification mechanism, have been accepted as key proteins in the safety transportation of metallic ions in plants. While the HIPPs gene family has been investigated in these model plants, such as Oryza sativa L. and Arabidopsis thaliana L., to our knowledge the HIPPs gene family in Brassica napus L remains analyzed. In this study, 104 HIPPs genes were identified in the genome of Brassica napus L. (Darmor-bzh v10) using Bioinformatics methods. The HIPPs members were not evenly distributed on 19 chromosomes. The amino acid number, protein molecular weight, isoelectric point, and protein hydrophobicity index of BnaHIPPs were ranged from 120 to 630 aa, 13 827.56 to 64 467.05 Da, 8.07, and -1.146 to -0.17, respectively. The HIPPs genes showed tissue specific expression and temporal specificity, as well as the highest transcripts in roots. Moreover, the expression of BnaHIPPs was significantly altered in response to Cd2+ stress treatment. For example, BnaA08p24150 and BnaA09p41730 were up-regulated in both roots and leaves. The phylogenetic analysis showed that BnaHIPPs were divided into five distinct clades. The collinearity analysis elucidated that the expansion of the BnaHIPPs gene family members was largely contributed by fragment replication, and only for four members BnaC05p3800, BnaC05p3810, BnaC05p3820, and BnaC05p3830 were found with tandem repetition. Our results will provided insights for future deciphering the BnaHIPPs gene family members in B. napus L..